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Protein Characterization and Purification Services

Recombinant Proteins Affinity chromatography: A powerful feature of recombinant DNA technology is the ability to add a purification “tag” to the protein. Under the most favorable conditions, highly purified protein will be isolated in a single chromatography step. If the future use of the protein prohibits a purification tag, other affinity chromatography methods are available at ATG Laboratories. You will receive the isolated protein with a detailed project report including…

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E. coli Expression Services

Subcloning/PCR Cloning ATG Laboratories will clone your insert into an expression vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the expression vector. Clones will be screened for the correct restriction fragments and sequenced, if necessary. A mid-scale plasmid preparation will be carried out on one correct clone (50-100 ml culture volume). You will receive the purified plasmid DNA, a…

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Baculovirus Expression Services

Subcloning/PCR Cloning ATG Laboratories will clone your insert into a baculovirus1 transfer vector. Either a standard subcloning or a PCR cloning will be used to transfer the gene of interest into the vector. Clones will be screened for the correct restriction fragments and sequenced, if necessary. A mid-scale plasmid preparation will be carried out on one correct clone (50-100 ml culture volume). You will receive the purified plasmid DNA, a…

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Cloning Services

Subcloning ATG Laboratories will clone your insert into a plasmid vector of your choice. Plasmid vector and insert DNA will be prepared by our personnel. After transformation, clones will be screened for the correct restriction fragments and for orientation, if necessary. A mid-scale plasmid preparation will be carried out on one correct clone (50-100 ml culture volume). You will receive the purified plasmid DNA, a stab culture of the correct…

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